Proteins are the important building blocks for tissues, organs, cells, enzymes, hormones and antibodies, and are indispensable for maintenance, development, reproduction and restore. By cultivating a particular microbial strain developed for the production of each amino acid, in a medium containing glucose or sugar and other vitamins (ammonium sulphate, and so forth., as nitrogen sources, minerals and nutritional vitamins), an amino acid might be effectively produced. Amino acids should be provided either in the form of protein or crystalline amino acids in feed to fulfill necessities.

They found that no regular cerevisiae or carlsbergensis strains utilised lysine whereas many different yeasts, together with wild yeasts, did so. They saved their stock cultures on malt extract agar slopes or on malt extract chalk agar within the case of Brettanomyces species. Later, Morris & Eddy1 described a strong lysine medium for the isolation and enumeration of wild yeasts in pitching yeast.

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