They discovered that no normal cerevisiae or carlsbergensis strains utilised lysine whereas many other yeasts, including wild yeasts, did so. They saved their stock cultures on malt extract agar slopes or on malt extract chalk agar in the case of Brettanomyces species. Later, Morris & Eddy1 described a stable lysine medium for the isolation and lawyer article enumeration of wild yeasts in pitching yeast.
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